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Of 32. The supernatant was treated with ammonium sulfate. Proteins that were precipitated in a range of ammonium sulfate concentrations between 0.24 and 0.31 g/ml were dissolved, dialyzed against Buffer A, and loaded onto a HiTrap heparin column (GE Healthcare). The flow-through fraction was repeatedly loaded onto an ATP-agarose (SigmaAldrich) column that was equilibrated with Buffer A containing

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