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Utants were purified from KY1603 cells harboring pET3a derivatives essentially as described (10), except that 32 synthesis was induced by a recombinant phage CE6 carrying the T7 RNA polymerase gene (Novagen) and that Buffer A (50 mM Tris-HCl (pH 7.5), 1 mM EDTA, 1 mM dithiothreitol, 10 (v/v) glycerol) was used in all steps. DnaK was purified from HB101 cells harboring pKV1957 (pKV1142 trcp-dnaK).

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